67.4 Folate:B12

67.4.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

Prolactin
Chisq Df Pr(>Chisq)
(Intercept) 250.7924480 1 <0.0001 ***
Study 3.2136720 1 0.07 ns
f39_gdm_new 0.5754347 1 0.4 ns
BMI 3.3368238 1 0.07 ns
Ethnicity_cauc 105.4219556 1 <0.0001 ***
f10c_any_cig_1st_vst 49.1590682 1 <0.0001 ***
GA_samp 164.6323095 1 <0.0001 ***
Study:f39_gdm_new 1.8652579 1 0.2 ns
HPL
Chisq Df Pr(>Chisq)
(Intercept) 150.8759535 1 <0.0001 ***
Study 7.3986291 1 0.007 **
f39_gdm_new 0.4357432 1 0.5 ns
BMI 236.2094019 1 <0.0001 ***
Ethnicity_cauc 5.5165373 1 0.02 *
f10c_any_cig_1st_vst 0.9176455 1 0.3 ns
GA_samp 2601.9722143 1 <0.0001 ***
Study:f39_gdm_new 1.4845286 1 0.2 ns
GH2
Chisq Df Pr(>Chisq)
(Intercept) 251.096669 1 <0.0001 ***
Study 1.107622 1 0.3 ns
f39_gdm_new 1.386104 1 0.2 ns
BMI 87.622496 1 <0.0001 ***
Ethnicity_cauc 3.246832 1 0.07 ns
f10c_any_cig_1st_vst 1.039153 1 0.3 ns
GA_samp 1032.266869 1 <0.0001 ***
Study:f39_gdm_new 3.376951 1 0.07 ns
Insulin
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 21.326 21.326 <0.0001 ***
3 f39_gdm_new 1 Inf 33.726 33.726 <0.0001 ***
4 BMI 1 Inf 330.722 330.722 <0.0001 ***
5 Ethnicity_cauc 1 Inf 25.046 25.046 <0.0001 ***
6 f10c_any_cig_1st_vst 1 Inf 3.158 3.158 0.08 ns
7 GA_samp 1 Inf 2.506 2.506 0.1 ns
2 Study:f39_gdm_new 1 Inf 4.089 4.089 0.04 *
HOMA.IR
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 36.051 36.051 <0.0001 ***
3 f39_gdm_new 1 Inf 32.027 32.027 <0.0001 ***
4 BMI 1 Inf 316.807 316.807 <0.0001 ***
5 Ethnicity_cauc 1 Inf 24.882 24.882 <0.0001 ***
6 f10c_any_cig_1st_vst 1 Inf 2.618 2.618 0.1 ns
7 GA_samp 1 Inf 1.602 1.602 0.2 ns
2 Study:f39_gdm_new 1 Inf 4.361 4.361 0.04 *
GLUC3_mmol.L
model term df1 df2 F.ratio Chisq p.value
1 Study 1 Inf 53.182 53.182 <0.0001 ***
3 f39_gdm_new 1 Inf 4.345 4.345 0.04 *
4 BMI 1 Inf 11.449 11.449 0.0007 ***
5 Ethnicity_cauc 1 Inf 0.718 0.718 0.4 ns
6 f10c_any_cig_1st_vst 1 Inf 0.028 0.028 0.9 ns
7 GA_samp 1 Inf 0.278 0.278 0.6 ns
2 Study:f39_gdm_new 1 Inf 1.558 1.558 0.2 ns

67.4.2 Model summary

Ratio of Geometric means (95% CI)

Prolactin
Var Effect P
(Intercept) 2.75 (2.39, 3.11) <0.0001
folate_B12 -0.00 (-0.00, 0.00) 0.08
f39_gdm_newGDM -0.10 (-0.33, 0.13) 0.38
StudySTOP -0.07 (-0.20, 0.05) 0.25
GA_samp 0.14 (0.12, 0.16) <0.0001
folate_B12:f39_gdm_newGDM 0.00 (-0.00, 0.00) 0.79
f39_gdm_newGDM:StudySTOP 0.16 (-0.07, 0.38) 0.17
folate_B12:StudySTOP 0.00 (-0.00, 0.00) 0.09
HPL
Var Effect P
(Intercept) 0.64 (0.46, 0.83) <0.0001
folate_B12 -0.00 (-0.00, 0.00) 0.71
f39_gdm_newGDM -0.07 (-0.18, 0.04) 0.24
StudySTOP 0.11 (0.04, 0.18) 0.001
GA_samp 0.29 (0.28, 0.30) <0.0001
folate_B12:f39_gdm_newGDM -0.00 (-0.00, 0.00) 0.84
f39_gdm_newGDM:StudySTOP 0.06 (-0.05, 0.18) 0.27
folate_B12:StudySTOP -0.00 (-0.00, 0.00) 0.53
GH2
Var Effect P
(Intercept) -2.55 (-2.80, -2.30) <0.0001
folate_B12 -0.00 (-0.00, -0.00) 0.01
f39_gdm_newGDM -0.16 (-0.32, 0.01) 0.06
StudySTOP -0.03 (-0.12, 0.06) 0.48
GA_samp 0.26 (0.25, 0.28) <0.0001
folate_B12:f39_gdm_newGDM 0.00 (-0.00, 0.00) 0.77
f39_gdm_newGDM:StudySTOP 0.17 (0.00, 0.33) 0.05
folate_B12:StudySTOP 0.00 (-0.00, 0.00) 0.62
Insulin
Var Effect P
(Intercept) 2.39 (1.93, 2.85) <0.0001
folate_B12 0.00 (-0.00, 0.00) 0.87
f39_gdm_newGDM 0.79 (0.34, 1.23) 0.0005
StudySTOP -0.19 (-0.36, -0.02) 0.03
GA_samp 0.02 (-0.01, 0.05) 0.20
folate_B12:f39_gdm_newGDM -0.00 (-0.00, 0.00) 0.98
f39_gdm_newGDM:StudySTOP -0.28 (-0.76, 0.21) 0.27
folate_B12:StudySTOP -0.00 (-0.00, 0.00) 0.25
folate_B12:f39_gdm_newGDM:StudySTOP 0.00 (-0.00, 0.00) 0.94
HOMA.IR
Var Effect P
(Intercept) 1.02 (0.53, 1.50) <0.0001
folate_B12 0.00 (-0.00, 0.00) 0.89
f39_gdm_newGDM 0.91 (0.41, 1.40) 0.0003
StudySTOP -0.34 (-0.52, -0.16) 0.0003
GA_samp 0.02 (-0.01, 0.05) 0.32
folate_B12:f39_gdm_newGDM -0.00 (-0.00, 0.00) 0.81
f39_gdm_newGDM:StudySTOP -0.36 (-0.90, 0.19) 0.20
folate_B12:StudySTOP -0.00 (-0.00, 0.00) 0.25
folate_B12:f39_gdm_newGDM:StudySTOP 0.00 (-0.00, 0.00) 0.84
GLUC3_mmol.L
Var Effect P
(Intercept) 1.72 (1.62, 1.81) <0.0001
folate_B12 0.00 (-0.00, 0.00) 0.94
f39_gdm_newGDM 0.14 (0.01, 0.27) 0.03
StudySTOP -0.15 (-0.18, -0.11) <0.0001
GA_samp -0.00 (-0.01, 0.00) 0.52
folate_B12:f39_gdm_newGDM -0.00 (-0.00, 0.00) 0.18
f39_gdm_newGDM:StudySTOP -0.08 (-0.19, 0.04) 0.18
folate_B12:StudySTOP 0.00 (-0.00, 0.00) 0.57

67.4.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Folate:B12 ratio and may be misleading when interaction term is involved.

Prolactin
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 97.20412 7.281342 86.51760 83.75653 112.8108
STOP non-GDM 95.58684 6.831944 71.92643 82.89315 110.2243
SCOPE GDM 88.72606 10.976535 527.10852 69.58321 113.1352
STOP GDM 102.20950 8.817559 148.76685 86.18988 121.2066
HPL
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 89.39632 2.386189 154.4630 84.80467 94.23659
STOP non-GDM 98.68489 2.406359 109.2051 94.02907 103.57123
SCOPE GDM 83.02221 4.493204 800.7892 74.65469 92.32759
STOP GDM 97.59695 3.506629 407.6971 90.94143 104.73956
GH2
Study f39_gdm_new response SE df lower.CL upper.CL
SCOPE non-GDM 2.349434 0.0669864 371.0951 2.221338 2.484917
STOP non-GDM 2.302558 0.0555231 236.1645 2.195732 2.414582
SCOPE GDM 2.025946 0.1537443 1373.2989 1.745722 2.351151
STOP GDM 2.343037 0.0992274 751.0354 2.156119 2.546160
Insulin
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 14.12243 0.6925753 Inf 12.82820 15.54723
STOP non-GDM 11.08270 0.4161006 Inf 10.29644 11.92900
SCOPE GDM 30.92614 4.9114459 Inf 22.65398 42.21889
STOP GDM 18.56835 1.4196529 Inf 15.98433 21.57011
HOMA.IR
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 3.418868 0.1780292 Inf 3.087152 3.786226
STOP non-GDM 2.307994 0.0911998 Inf 2.135992 2.493846
SCOPE GDM 8.231226 1.4380393 Inf 5.844603 11.592417
STOP GDM 4.003332 0.3292095 Inf 3.407407 4.703479
GLUC3_mmol.L
Study f39_gdm_new response SE df asymp.LCL asymp.UCL
SCOPE non-GDM 5.425348 0.0505319 Inf 5.327206 5.525298
STOP non-GDM 4.708502 0.0360794 Inf 4.638316 4.779750
SCOPE GDM 6.079494 0.3285876 Inf 5.468412 6.758863
STOP GDM 4.880484 0.0623350 Inf 4.759827 5.004201

67.4.4 Comparisons

67.4.4.1 Within Groups

Effect for every 100 unit increase in Folate:B12 ratio.
Hormone Contrast Study f39_gdm_new Ratio of Geometric means (95% CI) P-value
Prolactin folate_B12245.96 / folate_B12145.96 SCOPE non-GDM 0.979 (0.956, 1.003) 0.08
Prolactin folate_B12245.96 / folate_B12145.96 STOP non-GDM 1.008 (0.983, 1.033) 0.5
Prolactin folate_B12245.96 / folate_B12145.96 SCOPE GDM 0.985 (0.937, 1.036) 0.6
Prolactin folate_B12245.96 / folate_B12145.96 STOP GDM 1.014 (0.973, 1.057) 0.5
HPL folate_B12245.96 / folate_B12145.96 SCOPE non-GDM 0.998 (0.985, 1.010) 0.7
HPL folate_B12245.96 / folate_B12145.96 STOP non-GDM 0.992 (0.978, 1.005) 0.2
HPL folate_B12245.96 / folate_B12145.96 SCOPE GDM 0.995 (0.969, 1.022) 0.7
HPL folate_B12245.96 / folate_B12145.96 STOP GDM 0.989 (0.967, 1.012) 0.3
GH2 folate_B12245.96 / folate_B12145.96 SCOPE non-GDM 0.978 (0.961, 0.996) 0.01
GH2 folate_B12245.96 / folate_B12145.96 STOP non-GDM 0.984 (0.966, 1.003) 0.1
GH2 folate_B12245.96 / folate_B12145.96 SCOPE GDM 0.983 (0.947, 1.021) 0.4
GH2 folate_B12245.96 / folate_B12145.96 STOP GDM 0.990 (0.959, 1.021) 0.5
Insulin folate_B12245.96 / folate_B12145.96 SCOPE non-GDM 1.003 (0.967, 1.040) 0.9
Insulin folate_B12245.96 / folate_B12145.96 STOP non-GDM 0.976 (0.949, 1.004) 0.09
Insulin folate_B12245.96 / folate_B12145.96 SCOPE GDM 1.002 (0.913, 1.099) 1.0
Insulin folate_B12245.96 / folate_B12145.96 STOP GDM 0.979 (0.927, 1.034) 0.4
HOMA.IR folate_B12245.96 / folate_B12145.96 SCOPE non-GDM 1.003 (0.965, 1.041) 0.9
HOMA.IR folate_B12245.96 / folate_B12145.96 STOP non-GDM 0.975 (0.946, 1.004) 0.09
HOMA.IR folate_B12245.96 / folate_B12145.96 SCOPE GDM 0.987 (0.879, 1.109) 0.8
HOMA.IR folate_B12245.96 / folate_B12145.96 STOP GDM 0.973 (0.918, 1.033) 0.4
GLUC3_mmol.L folate_B12245.96 / folate_B12145.96 SCOPE non-GDM 1.000 (0.994, 1.006) 0.9
GLUC3_mmol.L folate_B12245.96 / folate_B12145.96 STOP non-GDM 1.004 (0.995, 1.012) 0.4
GLUC3_mmol.L folate_B12245.96 / folate_B12145.96 SCOPE GDM 0.986 (0.961, 1.010) 0.3
GLUC3_mmol.L folate_B12245.96 / folate_B12145.96 STOP GDM 0.989 (0.973, 1.006) 0.2

67.4.4.2 Between Groups

Below are comparisons of the differences in the change in Folate:B12 ratio between groups. I.e. whether the change in Folate:B12 ratio are different between groups.

Prolactin
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.059647 0.0365028 2086.782 0.9904256 1.133706 0.093 ns
STOP/SCOPE (non-GDM) 1.029392 0.0177303 2086.782 0.9952013 1.064756 0.093 ns
STOP/SCOPE (GDM) 1.029392 0.0177303 2086.782 0.9952013 1.064756 0.093 ns
GDM/non-GDM (SCOPE) 1.006263 0.0239070 1986.639 0.9604531 1.054258 0.793 ns
GDM/non-GDM (STOP) 1.006263 0.0239070 1986.639 0.9604531 1.054258 0.793 ns
HPL
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 0.9885105 0.0182531 1977.580 0.9533537 1.024964 0.532 ns
STOP/SCOPE (non-GDM) 0.9942387 0.0091794 1977.580 0.9763983 1.012405 0.532 ns
STOP/SCOPE (GDM) 0.9942387 0.0091794 1977.580 0.9763983 1.012405 0.532 ns
GDM/non-GDM (SCOPE) 0.9974014 0.0126798 1827.563 0.9728405 1.022582 0.838 ns
GDM/non-GDM (STOP) 0.9974014 0.0126798 1827.563 0.9728405 1.022582 0.838 ns
GH2
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.012904 0.0260314 2086.194 0.9631187 1.065262 0.618 ns
STOP/SCOPE (non-GDM) 1.006431 0.0129325 2086.194 0.9813861 1.032115 0.618 ns
STOP/SCOPE (GDM) 1.006431 0.0129325 2086.194 0.9813861 1.032115 0.618 ns
GDM/non-GDM (SCOPE) 1.005274 0.0178169 1979.419 0.9709322 1.040830 0.767 ns
GDM/non-GDM (STOP) 1.005274 0.0178169 1979.419 0.9709322 1.040830 0.767 ns
Insulin
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 0.9513796 0.0568495 Inf 0.8462341 1.069590 0.404 ns
STOP/SCOPE (non-GDM) 0.9732677 0.0228460 Inf 0.9295049 1.019091 0.248 ns
STOP/SCOPE (GDM) 0.9775107 0.0537323 Inf 0.8776720 1.088706 0.679 ns
GDM/non-GDM (SCOPE) 0.9985106 0.0508678 Inf 0.9036274 1.103357 0.977 ns
GDM/non-GDM (STOP) 1.0028636 0.0313648 Inf 0.9432360 1.066261 0.927 ns
HOMA.IR
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 0.9584520 0.0681427 Inf 0.8337825 1.101762 0.551 ns
STOP/SCOPE (non-GDM) 0.9722000 0.0240018 Inf 0.9262774 1.020399 0.253 ns
STOP/SCOPE (GDM) 0.9858588 0.0657369 Inf 0.8650810 1.123499 0.831 ns
GDM/non-GDM (SCOPE) 0.9848630 0.0615982 Inf 0.8712394 1.113305 0.807 ns
GDM/non-GDM (STOP) 0.9986997 0.0337874 Inf 0.9346254 1.067167 0.969 ns
GLUC3_mmol.L
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0067462 0.0119709 Inf 0.9835551 1.030484 0.572 ns
STOP/SCOPE (non-GDM) 1.0033674 0.0059653 Inf 0.9917434 1.015128 0.572 ns
STOP/SCOPE (GDM) 1.0033674 0.0059653 Inf 0.9917434 1.015128 0.572 ns
GDM/non-GDM (SCOPE) 0.9853927 0.0108380 Inf 0.9643779 1.006865 0.181 ns
GDM/non-GDM (STOP) 0.9853927 0.0108380 Inf 0.9643779 1.006865 0.181 ns

67.4.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

67.4.6 Plots